"
Team:ESBS-Strasbourg/Transformation Heat shock
From 2008.igem.org
(Difference between revisions)
KatjaK (Talk | contribs)
(New page: * ligation or 10 ng plasmid * add 50-100 µL of competent bacteria * take on ice for 30 min * heat shock at 42 °C for 2 min * add 300 µL LB and incubated at 37°C for 45 min * plate)
Newer edit →
(New page: * ligation or 10 ng plasmid * add 50-100 µL of competent bacteria * take on ice for 30 min * heat shock at 42 °C for 2 min * add 300 µL LB and incubated at 37°C for 45 min * plate)
Newer edit →
Revision as of 08:21, 5 August 2008
- ligation or 10 ng plasmid
- add 50-100 µL of competent bacteria
- take on ice for 30 min
- heat shock at 42 °C for 2 min
- add 300 µL LB and incubated at 37°C for 45 min
- plate
