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Team:Hawaii/Notebook/2008-08- 4
From 2008.igem.org
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Revision as of 21:15, 5 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid Prep
- Margaret
- I14032, oriT
Quantification of Parts with Gel
- Margaret
- I14032, oriT, L51020, pSB1A7, B015, B0034
PCR
- Margaret
- Rep, oriV, verification of I14032, oriT(just to make sure)
Transformation
- Margaret
- pSMC121 (we were running out of template)
Re-streak Library
- Margaret
- pSB1A7, L51020, psb3k3, psb1a3, oriT, psb1a2, db3.1 strain, i14032
Construction of p+r, g+t
- Grace
- Gel purified nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA PCR products
- RE digest
- nir, GFP, GFPf, I14032 digested with EcoRI and SpeI
- E0240 digested with EcoRI and PstI
- slr1, slr2, pilA digested with PstI and XbaI
- Ran RE digests on gel
- Incomplete digestion of nir, slr1, slr2, pilA
- Krystle
- Gel purified RE digests of nir1, GFPf1, GFP, E0240, I14032, slr1, slr2, pilA
- Ligated at 37C overnight:
- nir + B0030
- I14032 + B0030
- GFP + B0015
- GFPf + B0015
- Supposed to ligate at 25C
PCR
- Grace
- Redid Saturday's PCR of BB-pRl1383a and pRL1383a alongside test of PCR contamination
- Ran on 2.5% gel for 1 hour at 95V
- Gel doesn't match that from Saturday
- Redid PCR again to verify Saturday/today's PCR
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
