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EPF-Lausanne/6 August 2008
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| + | Miniprep of last part that had failed: I1466 | ||
| + | |||
| + | Digested E1010 and F1060 and purified for ligation | ||
| + | |||
| + | Simulation: Did a little. We have to find a way to minimise feedback. | ||
| + | |||
| + | Research hasn't yet found anything good. | ||
| + | Looking at a few ideas: | ||
| + | -Protease to degrade when state has been chosen | ||
| + | -RhlR mutation | ||
| + | -low detect to build in a time delay. | ||
Revision as of 15:33, 6 August 2008
Miniprep of last part that had failed: I1466
Digested E1010 and F1060 and purified for ligation
Simulation: Did a little. We have to find a way to minimise feedback.
Research hasn't yet found anything good. Looking at a few ideas: -Protease to degrade when state has been chosen -RhlR mutation -low detect to build in a time delay.
