"
Team:ESBS-Strasbourg/4 August 2008
From 2008.igem.org
(Difference between revisions)
(→WetLab) |
|||
| Line 10: | Line 10: | ||
* ligation into the XbaI, PstI cutted vector pSB1A2 ''(using protocol 2c)'' | * ligation into the XbaI, PstI cutted vector pSB1A2 ''(using protocol 2c)'' | ||
* transformation into competent TOP10 and plating on LB-Amp ''(using the heat-shock protocol)'' | * transformation into competent TOP10 and plating on LB-Amp ''(using the heat-shock protocol)'' | ||
| + | <br> | ||
| + | |||
| + | ''Robin'' <br> | ||
| + | - PCRs of Cin8, Tup1, Cln2, Hsl1, Cyc100-16 <br> | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 16:08, 6 August 2008
Contents |
DryLab
Modeling
WetLab
Katja
Construction of the operator BioBricks (GAL4O, LexAO, LacO, CIO, TetO)
- hybridisation of the ordered oligos
- ligation into the XbaI, PstI cutted vector pSB1A2 (using protocol 2c)
- transformation into competent TOP10 and plating on LB-Amp (using the heat-shock protocol)
Robin
- PCRs of Cin8, Tup1, Cln2, Hsl1, Cyc100-16
