Alberta NINT/31 July 2008
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(New page: < Back to notebook < Previous entry lab work (JD): Counted colonies on both mine and Shannon's plates from ...) |
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-my plate had 100s on the negative control so I assumed the vector was not cut properly | -my plate had 100s on the negative control so I assumed the vector was not cut properly | ||
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | ||
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+ | [[Alberta_NINT/1_August_2008 | Next entry >]] |
Revision as of 18:16, 6 August 2008
lab work (JD):
Counted colonies on both mine and Shannon's plates from yesterday's ligations -unfortunately equal numbers on negative plates and ligation plates so ligation did not work -my plate had 100s on the negative control so I assumed the vector was not cut properly Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel