Imperial College/10 August 2008

From 2008.igem.org

(Difference between revisions)
m
Line 1: Line 1:
* WETLAB TEAM
* WETLAB TEAM
-
   
+
#Begin culturing ''B.subtilis''
-
 
+
#Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015 and BBa_c0012
-
 
+
#Design all primers for PCR cloning
-
 
+
#Design primers for all the genomic ''B.subtilis'' sequences being synthesised at GeneArt '''just incase'''
 +
#PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome
 +
#Prepare ''B.subtilis'' for first microscopy session - a set of dilutions to ascertain optimal amount of cells per mL
 +
#Chris and James will attend microscope training
 +
#Produce cell number against OD<sub>600nm</sub> calibration curve for use in characterisation
* DRYLAB TEAM
* DRYLAB TEAM

Revision as of 18:01, 7 August 2008

  • WETLAB TEAM
  1. Begin culturing B.subtilis
  2. Transform XL1-blue E.coli with Biobricks containing BBa_B0015 and BBa_c0012
  3. Design all primers for PCR cloning
  4. Design primers for all the genomic B.subtilis sequences being synthesised at GeneArt just incase
  5. PCR clone AmyE from pDR111 and XylR from the B.subtilis genome
  6. Prepare B.subtilis for first microscopy session - a set of dilutions to ascertain optimal amount of cells per mL
  7. Chris and James will attend microscope training
  8. Produce cell number against OD600nm calibration curve for use in characterisation
  • DRYLAB TEAM
  1. Finish up tutorial 2 by this week
  2. Start working on tutorial 3
  3. Attend microscope training
  4. Generate data sets from bacteria motility movies
Retrieved from "http://2008.igem.org/Imperial_College/10_August_2008"