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Imperial College/10 August 2008
From 2008.igem.org
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#Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015 and BBa_c0012 | #Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015 and BBa_c0012 | ||
#Design all primers for PCR cloning | #Design all primers for PCR cloning | ||
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#PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome | #PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome | ||
#Prepare ''B.subtilis'' for first microscopy session - a set of dilutions to ascertain optimal amount of cells per mL | #Prepare ''B.subtilis'' for first microscopy session - a set of dilutions to ascertain optimal amount of cells per mL | ||
Revision as of 18:02, 7 August 2008
- WETLAB TEAM
- Begin culturing B.subtilis
- Transform XL1-blue E.coli with Biobricks containing BBa_B0015 and BBa_c0012
- Design all primers for PCR cloning
- PCR clone AmyE from pDR111 and XylR from the B.subtilis genome
- Prepare B.subtilis for first microscopy session - a set of dilutions to ascertain optimal amount of cells per mL
- Chris and James will attend microscope training
- Produce cell number against OD600nm calibration curve for use in characterisation
- DRYLAB TEAM
- Finish up tutorial 2 by this week
- Start working on tutorial 3
- Attend microscope training
- Generate data sets from bacteria motility movies
