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Imperial College/10 August 2008
From 2008.igem.org
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*Check successfulness of the linear DNA transformation, | *Check successfulness of the linear DNA transformation, | ||
| - | Produce cell number against OD<sub>600nm</sub> calibration curve for use in characterisation<font color=red> Do we want to do this next week or the following week? </font> | + | Produce cell number against OD<sub>600nm</sub> calibration curve for use in characterisation<font color=red> Do we want to do this next week or the following week? </font> <font color=blue>A possible activity for Friday if Wednesday's transformation is found to have failed?</font> |
===Cloning=== | ===Cloning=== | ||
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Chris and Tom for this week | Chris and Tom for this week | ||
| - | + | '''Monday''' | |
*Design all primers for PCR cloning | *Design all primers for PCR cloning | ||
| - | + | '''Tuesday''' | |
*Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015 and BBa_C0012 | *Transform XL1-blue ''E.coli'' with Biobricks containing BBa_B0015 and BBa_C0012 | ||
| - | + | '''Wednesday to Friday''' | |
*PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome when primers arrive | *PCR clone AmyE from pDR111 and XylR from the ''B.subtilis'' genome when primers arrive | ||
| Line 39: | Line 39: | ||
===Microscope=== | ===Microscope=== | ||
| - | + | *Prepare ''B.subtilis'' for first microscopy session on thursday - a set of dilutions to ascertain optimal amount of cells per mL | |
#Chris, James, Prudence and Clinton will attend microscope training | #Chris, James, Prudence and Clinton will attend microscope training | ||
Revision as of 09:28, 8 August 2008
Contents |
WETLAB TEAM
B.subtilis
Team members - James and Krupa + more if work load is too much.
Monday
- Prepare an overnight culture of DL1-blue E.coli with the pDR110 integration vector,
- Prepare an 2x overnight culture of B.subtilis,
- Prepare the reagents required for transformation,
Tuesday
- Miniprep of the DL1-blye overnight culture,
- Make competent cells for both of the transformation protocols
Wednesday
- Perform transformation of the competent cells using both protocols,
Thursday
- Check successfulness of the transformation. If successful then perform transformation of linear DNA using previously made competent cells.
Friday
- Check successfulness of the linear DNA transformation,
Produce cell number against OD600nm calibration curve for use in characterisation Do we want to do this next week or the following week? A possible activity for Friday if Wednesday's transformation is found to have failed?
Cloning
Chris and Tom for this week
Monday
- Design all primers for PCR cloning
Tuesday
- Transform XL1-blue E.coli with Biobricks containing BBa_B0015 and BBa_C0012
Wednesday to Friday
- PCR clone AmyE from pDR111 and XylR from the B.subtilis genome when primers arrive
- Prepare ALL remaining Protocols
- Depending on arrival of primers, connect XylR and BBa_C0012 to BBa_B0015 (separately) to begin construction of inducible promoters
Microscope
- Prepare B.subtilis for first microscopy session on thursday - a set of dilutions to ascertain optimal amount of cells per mL
- Chris, James, Prudence and Clinton will attend microscope training
DRYLAB TEAM
- Finish up tutorial 2 by this week
- Start working on tutorial 3
- Attend microscope training
- Generate data sets from bacteria motility movies
