Team:BCCS-Bristol/Calendar-Notebook/4 July 2008

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==Swimming agar assay==
==Swimming agar assay==
We wanted to test different conditions for the swimming agar assay:  
We wanted to test different conditions for the swimming agar assay:  

Revision as of 10:10, 8 August 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous



Swimming agar assay

We wanted to test different conditions for the swimming agar assay:

- different agar concentrations

- different incubation temperatures

- different inoculation depths

After an incubation overnight, we measured the diameter of the E. coli MG1655 swarms. Surprisingly, we observed two swarm layers: The lower one has a bigger diameter than the upper.

Agar (%) Temperature ( o C) Inoculation Depth Number JPG File Inner Ring (cm) Outer Ring (cm)
0.330Deep148284.65.3
0.330Deep248274.65.3
0.330Deep348294.75.2
0.330Under Surface148304.65.2
0.330Under Surface248314.65.2
0.330Under Surface348324.65.1
0.330On Surface148332.93
0.330On Surface248343.84.4
0.330On Surface348354.44.9
0.337.5Under Surface148367.27.6
0.337.5Under Surface248377.47.7
0.337.5Under Surface348387.17.4
0.325Under Surface148402.83.5
0.325Under Surface2483933.6
0.325Under Surface348413.13.7
0.230Under Surface148428.68.6
0.230Under Surface248438.68.6
0.230Under Surface348448.68.6
0.530Under Surface148472.32.3
0.530Under Surface248451.91.9
0.530Under Surface348461.51.5
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