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Team:BCCS-Bristol/Calendar-Notebook/17 July 2008
From 2008.igem.org
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==Comparision of motility between ''E. coli'' MG1655 and MC1000 (3rd attempt)== | ==Comparision of motility between ''E. coli'' MG1655 and MC1000 (3rd attempt)== | ||
| - | The experiment (compare [Team:BCCS-Bristol/Calendar-Notebook/15 July 2008 15 July 2008] and [Team:BCCS-Bristol/Calendar-Notebook/16 July 2008 16 July 2008]) was repeated with sightly different conditions. This time, there were no antibiotics in the agar for the motility comparison between the two strains and the bacteria were inoculated in a well made by a puncher. | + | The experiment (compare [[Team:BCCS-Bristol/Calendar-Notebook/15 July 2008 | 15 July 2008]] and [[Team:BCCS-Bristol/Calendar-Notebook/16 July 2008 | 16 July 2008]]) was repeated with sightly different conditions. This time, there were no antibiotics in the agar for the motility comparison between the two strains and the bacteria were inoculated in a well made by a puncher. |
Results of motility comparison showed that MG1655 had greater motility than MC1000, this was still not at all what we expected. | Results of motility comparison showed that MG1655 had greater motility than MC1000, this was still not at all what we expected. | ||
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| - | A 20% aspartic acid solution(as strong as we could get it) was made. | + | A 20 % aspartic acid solution (as strong as we could get it) was made. |
Revision as of 11:39, 8 August 2008
Comparision of motility between E. coli MG1655 and MC1000 (3rd attempt)
The experiment (compare 15 July 2008 and 16 July 2008) was repeated with sightly different conditions. This time, there were no antibiotics in the agar for the motility comparison between the two strains and the bacteria were inoculated in a well made by a puncher. Results of motility comparison showed that MG1655 had greater motility than MC1000, this was still not at all what we expected.
Miscellaneous
Some plates were made.
Some broth was innoculated for an overnight culture to measure the growth curve of MC1000 and MG1655 tomorrow.
| LB Broth 100ml | weight (g) |
| Yeast Extract | 0.5 |
| Tryptone | 1 |
| NaCl | 1 |
A 20 % aspartic acid solution (as strong as we could get it) was made.
