Team:NTU-Singapore/Notebook/27 May 2008

From 2008.igem.org

(Difference between revisions)
(Tuesday)
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*Choon Kit & Hung ([[User:Greenbear|Greenbear]])
*Choon Kit & Hung ([[User:Greenbear|Greenbear]])
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**Repeated the transformation of plasmids with Fe promoter after the first one yielded no cell growth :(
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**Repeated the transformation of plasmids with Fe promoter after the first one yielded no cell growth. This time, we used Ultracompetent TOP5 cells. :(
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**Inoculation of E coli that were successfully transformed with LacI (2 colonies grow).
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**Amplification of E coli that were successfully transformed with LacI (2 colonies grow).

Revision as of 03:05, 29 May 2008

Tuesday

  • Chin Chong (ChinChong)
    • Finalized Order-list for SBS store
    • Requested W3110 strain with luxS knockout from Princeton university
    • Canceled request from Yale
  • Zhen Fu
    • Come up with preliminary model for simulation
  • Min Lin
    • Finalized Sponsorship letter
    • Ordered contact cards for the group
  • Darius
    • Read through articles related to the project
    • Propose oligo nucleotides needed with the team
  • Choon Kit & Hung (Greenbear)
    • Repeated the transformation of plasmids with Fe promoter after the first one yielded no cell growth. This time, we used Ultracompetent TOP5 cells. :(
    • Amplification of E coli that were successfully transformed with LacI (2 colonies grow).