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Team:University of Sheffield /Project
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== '''Overall project''' == | == '''Overall project''' == | ||
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====Project Overview==== | ====Project Overview==== | ||
The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing. Quorum sensing systems use sensing proteins in the membrane (histidine-kinases in this case) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA, and in Legionella they are LqsS and LqsA. | The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing. Quorum sensing systems use sensing proteins in the membrane (histidine-kinases in this case) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA, and in Legionella they are LqsS and LqsA. | ||
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| + | Our organism would initially be sense Vibrio cholerae or Legionella. This will be done by hijacking a pathway already present in E.coli – the non-essential kdp pathway which is partly responsible for K+ detection and response regulation. This pathway has a similar histidine-kinase as its sensing molecule (KdpD) to Vibrio cholerae and Legionella, and results in a strong DNA response. The original KdpD will be knocked out of E.coli to make way for recombinant receptors. | ||
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====History of development==== | ====History of development==== | ||
*[[Team:University_of_Sheffield / Initial_BrSt |Early Brainstorming & Other Crazy Ideas]] | *[[Team:University_of_Sheffield / Initial_BrSt |Early Brainstorming & Other Crazy Ideas]] | ||
Revision as of 20:50, 8 August 2008
| Home | The Team | The Project | Calendar of Events | Notebook |
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Contents |
Overall project
Project Overview
The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing. Quorum sensing systems use sensing proteins in the membrane (histidine-kinases in this case) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA, and in Legionella they are LqsS and LqsA.
If everything goes well
Our organism would initially be sense Vibrio cholerae or Legionella. This will be done by hijacking a pathway already present in E.coli – the non-essential kdp pathway which is partly responsible for K+ detection and response regulation. This pathway has a similar histidine-kinase as its sensing molecule (KdpD) to Vibrio cholerae and Legionella, and results in a strong DNA response. The original KdpD will be knocked out of E.coli to make way for recombinant receptors.
History of development
- Early Brainstorming & Other Crazy Ideas
- A Selected Primary Idea: Sensing Pathogens & Biochip
- v1.0: A completed Design Proposal & Basic Model
- The final proposal: The hybrid-hybrid Sensor
Project Details
| Parts submitted to the Registry |
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| Modelling |
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