Team:Paris/August 11

From 2008.igem.org

(Difference between revisions)
(→Culture of ligation transformants)
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{|| Border="1"
{|| Border="1"
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|align="center"|'''Ligation'''
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|- style="text-align: center;"|'''Ligation'''
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| colspan="4" align="center"|L128
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| colspan="4"|L128
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| colspan="4" align="center"|L129
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| colspan="4"|L129
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| colspan="4" align="center"|L130
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| colspan="4"|L130
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|-
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|- style="text-align: center;"|'''Name'''
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|align="center"|'''Name'''
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| colspan="4"| pFlgA
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| colspan="4" align="center"|pFlgA
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| colspan="4"| pFlgB
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| colspan="4" align="center"|pFlgB
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| colspan="4"|pFlhB
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| colspan="4" align="center"|pFlhB
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|- style="text-align: center;"|'''Clone N°'''
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|-
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|1
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|align="center"|'''Clone N°'''
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|2
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|align="center"|1
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|3
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|align="center"|2
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|4
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|align="center"|3
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|1
-
|align="center"|4
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|2
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|align="center"|1
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|6
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|align="center"|2
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|7
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|align="center"|6
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|1
-
|align="center"|7
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|2
-
|align="center"|1
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|7
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|align="center"|2
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|8
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|align="center"|7
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|- style="text-align: center;"|'''Red fluorescence'''
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|align="center"|8
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-
|-
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-
|align="center"|'''Red fluorescence'''
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|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
-
|align="center"|no
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|no
-
|align="center"|no
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|no
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
-
|align="center"|no
+
|no
-
|align="center"|no
+
|no
|style="background: #ff6d73"|yes
|style="background: #ff6d73"|yes
-
|align="center"|no
+
|no
-
|align="center"|no
+
|no
-
|align="center"|no
+
|no
|}
|}

Revision as of 17:57, 11 August 2008

← Yesterday

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Tomorrow →

Culture of ligation transformants

  • 4 clones of each transformation were cultured in 7,5 mL LB + ampicilline. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
  • 37°C overnight
L128 L129 L130
pFlgA pFlgB pFlhB
1 2 3 4 1 2 6 7 1 2 7 8
yes yes no no yes yes no no yes no no no
Retrieved from "http://2008.igem.org/Team:Paris/August_11"