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Team:Montreal/Notebook/July
From 2008.igem.org
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=== July 1 === | === July 1 === | ||
*Maxi-prep of J-brick, assuming high copy number plasmid. | *Maxi-prep of J-brick, assuming high copy number plasmid. | ||
| + | |||
| + | === July 2 === | ||
| + | *Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated. | ||
| + | |||
| + | === July 3 === | ||
| + | *Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes. | ||
| + | |||
| + | === July 4 === | ||
| + | *Preparation of media. | ||
| + | |||
| + | === July 7 === | ||
| + | *Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin. | ||
| + | |||
| + | === July 8 === | ||
| + | *Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal. | ||
| + | |||
| + | === July 9 === | ||
| + | * | ||
Latest revision as of 19:52, 11 August 2008
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Contents |
July 2008
July 1
- Maxi-prep of J-brick, assuming high copy number plasmid.
July 2
- Gel of J-brick maxi-prep, results indicate large plasmid at well over 13K. Assumed incorrect, to be repeated.
July 3
- Repeat gel, of J-brick maxi prep from July 1st, negative results. Gel from July 2nd assumed contamination from other lanes.
July 4
- Preparation of media.
July 7
- Seeding of J-brick for mini-prep, 4x5mL cultures with ampicillin.
July 8
- Mini-prep of J-brick cultures followed by gel giving negative results - no DNA seen, but ladder appears normal.
