User:University of Washington/11 August 2008
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(New page: ==LuxR from AraC and TetR(Faifan)== -Miniprepped and sent sequence of ligated pSB1AC3 and promoter D29 (x3 of 25 degree Celsius ligation, x3 of 4 degree Celsius ligation) -Grew overnight...)
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(New page: ==LuxR from AraC and TetR(Faifan)== -Miniprepped and sent sequence of ligated pSB1AC3 and promoter D29 (x3 of 25 degree Celsius ligation, x3 of 4 degree Celsius ligation) -Grew overnight...)
Newer edit →
Revision as of 23:39, 11 August 2008
LuxR from AraC and TetR(Faifan)
-Miniprepped and sent sequence of ligated pSB1AC3 and promoter D29 (x3 of 25 degree Celsius ligation, x3 of 4 degree Celsius ligation)
-Grew overnight x3 from the single colony from QuikChange(trail#3) in Tsy + Amp
-Did Dpn1 reaction (continue from Ingrid's QuikChange#4), let sit from 3.5 hours, PCR purified and transformed into XL1-Blue (using 5ul DNA), grew on Amp plate.
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