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EPF-Lausanne/11 August 2008
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| + | =Molecular Biologie= | ||
| + | We try by different way to assemble biobrick | ||
| + | |||
| + | ==E1010 and F1610== | ||
| + | We try last week to assemble two big parts because it is easy to purify it. | ||
| + | We do ligation and transformation last week. The resultat was great all of the ten plates work. | ||
| + | Monday, we decide to do a mini prep of these cells to purifiy DNA. Then we want to check the palsmide so we digest a small quantity of them with EcoRI and SpeI to prepare it to do a gel tomorrow. | ||
| + | |||
| + | ==by Gel== | ||
| + | We try again to purifiy insert and vector by Gel. | ||
| + | We try to assemble : | ||
| + | R0040 and B0034 | ||
| + | R0071 and B0034 | ||
| + | I1466 and I1433 | ||
| + | We successful have isolate from Gel : R0040, R0071, B0034 | ||
| + | |||
http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase | http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase | ||
Revision as of 08:16, 12 August 2008
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Molecular Biologie
We try by different way to assemble biobrick
E1010 and F1610
We try last week to assemble two big parts because it is easy to purify it. We do ligation and transformation last week. The resultat was great all of the ten plates work. Monday, we decide to do a mini prep of these cells to purifiy DNA. Then we want to check the palsmide so we digest a small quantity of them with EcoRI and SpeI to prepare it to do a gel tomorrow.
by Gel
We try again to purifiy insert and vector by Gel. We try to assemble : R0040 and B0034 R0071 and B0034 I1466 and I1433 We successful have isolate from Gel : R0040, R0071, B0034
http://openwetware.org/wiki/Knight:Annealing_and_primer_extension_with_Klenow_polymerase
