Team:Paris/Modeling/f2

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Revision as of 18:41, 12 August 2008

No particular strain.

Exactly as for the binding of IPTG on LacR (see TF concentration control, leading to a lowered inhibition of Plac, aTc binds to TetR. So, after having determinated the Hill function [expr.Ptet] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.Ptet] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetR_active] = ƒ2bis(aTc) = f2^-1([expr.Ptet]), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis

Otherwise, if we find in the litterature the dissociation constant of the reaction

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 [[Image:f2.png]]
-Exactly as for the binding of IPTG on LacR [[Team:Paris/Modeling/estimation#how_to_control_transcription_factor_concentration]], leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = &#131;2(TetR) = &#131;2aux(IPTG), we can study [expr.''Ptet''] = &#131;2ter([TetR],[aTc]), and determine by calcul [TetR<sub>active</sub>] = &#131;2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of &#131;2ter = &#131;2&#959;&#131;2bis
+Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]], leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = &#131;2(TetR) = &#131;2aux(IPTG), we can study [expr.''Ptet''] = &#131;2ter([TetR],[aTc]), and determine by calcul [TetR<sub>active</sub>] = &#131;2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of &#131;2ter = &#131;2&#959;&#131;2bis
 [[Image:f2alt.png]]
 Otherwise, if we find in the litterature the dissociation constant of the reaction