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Team:Paris/Modeling/f2
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[[Image:f2.png]] | [[Image:f2.png]] | ||
| - | Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]], leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.''Ptet''] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetR<sub>active</sub>] = ƒ2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis | + | Exactly as for the binding of IPTG on LacR (see [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|TF concentration control]]), leading to a lowered inhibition of ''Plac'', aTc binds to TetR. So, after having determinated the Hill function [expr.''Ptet''] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.''Ptet''] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetR<sub>active</sub>] = ƒ2bis(aTc) = f2<sup>-1</sup>([expr.''Ptet'']), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis |
[[Image:f2alt.png]] | [[Image:f2alt.png]] | ||
Otherwise, if we find in the litterature the dissociation constant of the reaction | Otherwise, if we find in the litterature the dissociation constant of the reaction | ||
Revision as of 18:41, 12 August 2008
No particular strain.
Exactly as for the binding of IPTG on LacR (see TF concentration control), leading to a lowered inhibition of Plac, aTc binds to TetR. So, after having determinated the Hill function [expr.Ptet] = ƒ2(TetR) = ƒ2aux(IPTG), we can study [expr.Ptet] = ƒ2ter([TetR],[aTc]), and determine by calcul [TetRactive] = ƒ2bis(aTc) = f2-1([expr.Ptet]), to endly give an analytical expression of ƒ2ter = ƒ2οƒ2bis
Otherwise, if we find in the litterature the dissociation constant of the reaction
