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Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
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== GROUP 3: Short two way stops == | == GROUP 3: Short two way stops == | ||
| + | |||
| + | Date 8/12/08 | ||
| + | Status report by: Raghav and James | ||
| + | Part no.:BBa_K110011 -> BBa_K110013; BBa_K110010;17 | ||
| + | Part Description: | ||
| + | Summary:<b>Short two way stops should be sent out for sequencing this week</b> Needed | ||
| + | so much DNA to visualize one band of short two way stop. <b>Sapphire FP progress | ||
| + | has slowed</b>, due to extraneous bands in gel and extraneous sequence from the trace | ||
| + | files.<b> transformations from the biobrick book were tried again with very poor | ||
| + | results... 1 COLONY... POSSIBLY </b> THE BIOBRICK BOOK IS UPSETTING. | ||
| + | |||
| + | Date 8/5/08 | ||
| + | Status report by: James | ||
| + | Part no.: BBa_K110011 -> BBa_K110013; BBa_K110010;17 | ||
| + | Part Description: Short two way stops; and Sapphire Fluorescent Proteins | ||
| + | Summary: The sequence data from last week, turned out all right | ||
| + | <b>both orientations of Sapphire FP should be correct sequences | ||
| + | [[Media:JHU_Saph_Seq_1.zip|Sequence Data]] </b> HOORAY! | ||
| + | Last weeks miniprep and digestion yielded no DNA, so this week the minprep was repeated and the | ||
| + | digestion of the prodeuct can be found here<b> RE digest [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-8-5.Two%20way%20stop%20digestion%208/05/08.Jasper;%20James;%20Raghav.html Two way stop digestion 8/05/08]</b> | ||
| + | |||
| + | Date 7/29/08 | ||
| + | Status report by: James | ||
| + | Part no.: BBa_K110011 -> BBa_K110013; BBa_K110010;17 | ||
| + | Part Description: Short two way stops | ||
| + | Summary: Sequencing of BBa_K110010;17 <b>(Sapphire FP) revealed confusing, if not bad | ||
| + | sequences.[[Media:JHU_Saph_Seq_1.zip|Sequence Data]] </b> | ||
| + | PCR cloning and transformation was completed sucessfully last week on the Short two way stops | ||
| + | A Miniprep was done and a <b> RE digest will be completed soon (possibly tonight)</b> | ||
| + | |||
| + | Date: 7/22/2008 | ||
| + | Status report by: James | ||
| + | Part no.: BBa_K110011 -> BBa_K110013 | ||
| + | Part Description: Short two way stops | ||
| + | Summary: RE digestion showed no insert the plasmid of our clones, thus the PCR was done again. | ||
| + | [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-23.Short%20way%20stops.James.html Short way stops] | ||
Date: 7/21/08 | Date: 7/21/08 | ||
Revision as of 01:32, 13 August 2008
GROUP 3: Short two way stops
Date 8/12/08 Status report by: Raghav and James Part no.:BBa_K110011 -> BBa_K110013; BBa_K110010;17 Part Description: Summary:Short two way stops should be sent out for sequencing this week Needed so much DNA to visualize one band of short two way stop. Sapphire FP progress has slowed, due to extraneous bands in gel and extraneous sequence from the trace files. transformations from the biobrick book were tried again with very poor results... 1 COLONY... POSSIBLY THE BIOBRICK BOOK IS UPSETTING.
Date 8/5/08 Status report by: James Part no.: BBa_K110011 -> BBa_K110013; BBa_K110010;17 Part Description: Short two way stops; and Sapphire Fluorescent Proteins Summary: The sequence data from last week, turned out all right both orientations of Sapphire FP should be correct sequences Sequence Data HOORAY! Last weeks miniprep and digestion yielded no DNA, so this week the minprep was repeated and the digestion of the prodeuct can be found here RE digest [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-8-5.Two%20way%20stop%20digestion%208/05/08.Jasper;%20James;%20Raghav.html Two way stop digestion 8/05/08]
Date 7/29/08 Status report by: James Part no.: BBa_K110011 -> BBa_K110013; BBa_K110010;17 Part Description: Short two way stops Summary: Sequencing of BBa_K110010;17 (Sapphire FP) revealed confusing, if not bad sequences.Sequence Data PCR cloning and transformation was completed sucessfully last week on the Short two way stops A Miniprep was done and a RE digest will be completed soon (possibly tonight)
Date: 7/22/2008 Status report by: James Part no.: BBa_K110011 -> BBa_K110013 Part Description: Short two way stops Summary: RE digestion showed no insert the plasmid of our clones, thus the PCR was done again. [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-23.Short%20way%20stops.James.html Short way stops]
Date: 7/21/08 status report by: James Part no.: BBa_K110011 Part Description: Between-bud 27-W FRS2-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-22.Short%202Way%20Stop,%20Alpha%20Promoters,%20&%20Sapphire%20FP.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html Short 2Way Stop, Alpha Promoters, & Sapphire FP] Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep digestion yielded no product, PCR; ligation; transformation; and miniprep will be repeated by this Wednesday (07/23/08)
status report by: James Part no.: BBa_K110012 Part Description: Between STE2-W and BST1-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Yes [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-22.Short%202Way%20Stop,%20Alpha%20Promoters,%20&%20Sapphire%20FP.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html Short 2Way Stop, Alpha Promoters, & Sapphire FP] Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Current status of this part: Miniprep digestion yielded no product, PCR; ligation; transformation; and miniprep will be repeated by this Wednesday (07/23/08)
status report by: James Part no.: BBa_K110013 Part Description: Between-SWP82-W and EMP47-C LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; No Cloning of PCR product successful: No Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
