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EPF-Lausanne/12 August 2008
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=Molecular Biology, assemblage of biobricks= | =Molecular Biology, assemblage of biobricks= | ||
| + | All cells culture work but unfortunately, there is a problem in mini-prep procedure, any one have more than 30 ng/ul . So we do again new cell cultures. | ||
| + | |||
==F1610 and E1010== | ==F1610 and E1010== | ||
| - | We do a gel to see..... | + | |
| + | We do a gel to see if we can see fragments(we cut plasmids with SpeI and EcoRI to see size of insert). Unfortunately we see nothings, any fragment with 1500 bp. But the positive control doesn't work too. The positive control is F1610(800 bp) and E1010 (700 bp). So we decide to do tomorrow a test of digestion with only E1010 and F1610 that we use for positive control compare to a new digestion of these Biobricks that we do today. To see if there is problem in digestion procedure. | ||
== aa== | == aa== | ||
Revision as of 07:06, 13 August 2008
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Molecular Biology, assemblage of biobricks
All cells culture work but unfortunately, there is a problem in mini-prep procedure, any one have more than 30 ng/ul . So we do again new cell cultures.
F1610 and E1010
We do a gel to see if we can see fragments(we cut plasmids with SpeI and EcoRI to see size of insert). Unfortunately we see nothings, any fragment with 1500 bp. But the positive control doesn't work too. The positive control is F1610(800 bp) and E1010 (700 bp). So we decide to do tomorrow a test of digestion with only E1010 and F1610 that we use for positive control compare to a new digestion of these Biobricks that we do today. To see if there is problem in digestion procedure.
