Team:Paris/Modeling/f2

From 2008.igem.org

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[[Image:f2b.png]]
[[Image:f2b.png]]
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To deduce from our measurements the correct hill function, see the [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|different methods]].
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This interaction must be quite weak in comparison to the others, but nevertheless we would like to study it. We will consider that, as for many promoters induced by several transcription factors, a ''SUM'' logical gate synthetise the contribution.
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The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR&#8644; aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before ''tetR'' and of the added aTc. In this way, we just have to study [expr.''Ptet''] = &#131;2(TetR<sub>free</sub>).
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Revision as of 15:45, 13 August 2008

No particular strain.

F2b.png

This interaction must be quite weak in comparison to the others, but nevertheless we would like to study it. We will consider that, as for many promoters induced by several transcription factors, a SUM logical gate synthetise the contribution.

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