"
Team:KULeuven/13 August 2008
From 2008.igem.org
(Difference between revisions)
(New page: {{:Team:KULeuven/Tools/New_Day/Date_Retriever}} == Lab Work == === Wet Lab === * We tested the ligations (using PCR and gel electrophoresis) of the following parts: J23109 + J23032, I712...) |
m (→Wet Lab) |
||
| Line 6: | Line 6: | ||
* We tested the ligations (using PCR and gel electrophoresis) of the following parts: J23109 + J23032, I712074 + J23032, K124015 + B0015, R0084 + J23022, R0062 + B0032 and C0012+ B0015. They seemed to have ligated properly. After that, we digested these ligations for the next step in the ligation process. | * We tested the ligations (using PCR and gel electrophoresis) of the following parts: J23109 + J23032, I712074 + J23032, K124015 + B0015, R0084 + J23022, R0062 + B0032 and C0012+ B0015. They seemed to have ligated properly. After that, we digested these ligations for the next step in the ligation process. | ||
* We MiniPrepped the following parts: C0040 + B0015, C0060 + B0015 and J23100 + B0032. They will be tested tomorrow. | * We MiniPrepped the following parts: C0040 + B0015, C0060 + B0015 and J23100 + B0032. They will be tested tomorrow. | ||
| - | * We continued to make the T7 polymerase | + | * We continued to make the T7 polymerase with UmuD tag. Therefore we used PCR in two steps. The first step worked, but the second one didn't. There might be something wrong with the primer (it was a very long one). |
| - | + | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 20:26, 13 August 2008
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
- We tested the ligations (using PCR and gel electrophoresis) of the following parts: J23109 + J23032, I712074 + J23032, K124015 + B0015, R0084 + J23022, R0062 + B0032 and C0012+ B0015. They seemed to have ligated properly. After that, we digested these ligations for the next step in the ligation process.
- We MiniPrepped the following parts: C0040 + B0015, C0060 + B0015 and J23100 + B0032. They will be tested tomorrow.
- We continued to make the T7 polymerase with UmuD tag. Therefore we used PCR in two steps. The first step worked, but the second one didn't. There might be something wrong with the primer (it was a very long one).
