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Team:The University of Alberta/13 August 2008
From 2008.igem.org
(Difference between revisions)
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*Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks. | *Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks. | ||
*Digested RBS+TetR with Eco/XbaI. | *Digested RBS+TetR with Eco/XbaI. | ||
| + | |||
| + | '''Chris''' | ||
| + | *Jason and I began the site-directed mutagenesis of the binary vector. Its going to take a loooong time (over 7 hours for the PCR alone) so we are going to finish it (i.e. do the digestion with DpnI and transform) tomorrow. | ||
Latest revision as of 21:11, 13 August 2008
Saima
- Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks.
- Digested RBS+TetR with Eco/XbaI.
Chris
- Jason and I began the site-directed mutagenesis of the binary vector. Its going to take a loooong time (over 7 hours for the PCR alone) so we are going to finish it (i.e. do the digestion with DpnI and transform) tomorrow.
