Team:University of Lethbridge/Notebook/GeneralLabAugust
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(Difference between revisions)
(New page: ===Augest 13, 2008=== ====Nathun Puhl and Sebastian==== Varified and quantified the LacI and DT restriction digest products. Set up a Ligase experiment for LacI and DT --1.3 uL vector(DT...) |
(→Nathun Puhl and Sebastian) |
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Set up a Ligase experiment for LacI and DT | Set up a Ligase experiment for LacI and DT | ||
- | + | - 1.3 uL vector(DT) | |
- | + | - 8 uL insert(LacI) | |
- | + | - 2 uL 10x Ligase buffer | |
- | + | - 1.5 uL Ligase | |
- | + | - 7.2 uL ddH2O | |
- | + | - 20 uL total volume |
Revision as of 02:09, 14 August 2008
Augest 13, 2008
Nathun Puhl and Sebastian
Varified and quantified the LacI and DT restriction digest products.
Set up a Ligase experiment for LacI and DT
- 1.3 uL vector(DT) - 8 uL insert(LacI) - 2 uL 10x Ligase buffer - 1.5 uL Ligase - 7.2 uL ddH2O - 20 uL total volume