"
EPF-Lausanne/14 August 2008
From 2008.igem.org
(New page: [https://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_August_2008 <<Previous] - [https://2008.igem.org/Team:EPF-Lausanne/Notebook Back to Notebook] - [https://2008.igem.org/wiki/index....)
Newer edit →
Revision as of 16:04, 14 August 2008
<<Previous - Back to Notebook - Next>>
Molecular biology
We do mini-prep of E1010, "F1610" and P0140
E1010/F1610
Yesterday we find that F1610 make 300bp. So two hypothesis: 1) The mini-prep is bad or mix 2) the biobrick from IGEM is bad.
So we do another digestion serie with EcoRI and SpeI. We do with different mini-prep of F1610 and the last mini-prep of E1010 that work(for positiv control).
Some mini-prep from F1610 are too small, there isn't enough liquid so we do PCR with these (we use E1010 from last mini-prep as a positiv control).
We do in same time PCR of all fragments that ligated E1010/F1610 to see if there is a fragment of 1500 bp.
Resultat of Gel
All mini-preps of F1610 have a fragment of 300 bp. So the biobrick from IGEM doesn't work. All ligated fragments E1010/F1610 aren't ligate. (see p. 75 in notebook of lab)
