Team:University of Chicago/Meetings

From 2008.igem.org

Revision as of 22:26, 30 May 2008

Meetings

Hokay, so here's the easiest way I could imagine us keeping track of all our various meetings/appointments: a calendar page. I think it would be pretty easy to just list things in order of most recent first, along with who should be there and a brief description of what the meeting is about. You should also post anything important resulting from the meeting after it happens. For instance:

Monday - 26 May 2008 - 1:00PM - GCIS

Who should be there: Everyone. We'll be discussing the papers Steve sent out on May 19 (titled "Concepts"); hearing about E. coli secretion systems (Nora, Thomas); hearing about the Schultz method (Jata); and going over funding (Laura) and infrastructure (Jata, Damon).

File:052608notes.txt

Tuesday - 27 May 2008 - 12:30PM - C-Shop

Who should be there: Jata & Damon. We'll be discussing infrastructure, and our pending meeting with Dr. Schonbaum.

Sent Dr. Schonbaum an email:

   Dr Schonbaum,

   Thank you for agreeing to meet with Damon and I tomorrow in regards to our
   synthetic biology research over the summer;  we're very grateful for your
   help.  We're writing you this email to give you an idea of the things we'd
   like to cover when we see you.

   1.       Access: Over the summer, how will we get into BSLC, when and
   where are we allowed to go, who can we ask for access to things we need in
   areas we cannot go, and what do we need to do to obtain the keys/ID
   necessary to make things run smoothly?
   2.        Supplies: What supplies will we have regular access to, which
   need to be ordered, how do we order them, where do we store them, and how
   much do you recommend we keep in stock to perform the methods listed
   below? What can we do to ensure a smooth transition from summer research
   to continuing the research during the next school year?
   3.       Waste: safety, legal, and environmental concerns?
   4.       Equipment: what should we get;,what's entirely ours and what will
   be shared, will we need special access to the shared equipment if it's in
   another lab or operated by someone else (e.g., autoclave, microscopy,
   refrigeration)if we really need something that they won't give, how should
   we request or buy the equipment?
   5.        Safety: training, certification, paperwork; rules and where to
   get them?

   Here is a link to our proposed methods:
   https://2008.igem.org/Team:University_of_Chicago/Project

   We are working on beginning a collaboration with Phillip Messersmith
   (Departments of Biomedical Engineering and Materials Science and
   Engineering, Northwestern), who will hopefully assist us in the last
   (mussel foot protein specific) steps of our procedure; however, we'd like
   to figure out what can be done here and what must be done elsewhere.

   Thank you, and we look forward to meeting you tomorrow at 11:30AM!

    -Parijata Mackey and Damon Wang

Wednesday - 28 May 2008 - 11:30AM - BSLC 215

Who should be there: Jata & Damon. We'll be meeting with Dr. Schonbaum to discuss lab infrastructure for the summer.

Dr. Schonbaum will email us, but from memory,

• Basically no safety or legal issues
• All the usual molecular biology equipment and reagents will be available
• Officially in Rm 214 (?) but have 24hr(?) access to equipment in the neighboring labs and to reagents in Rm 209
• Keys to Rm 214 and Rm 209 for as many of everyone as Marcia has keys
• Schonbaum can give us His-tagged E. coli vectors
• Schonbaum teaches an all-day lab class for high school students beginning 23(?) June, so we should figure out what we need before then
• Schonbaum will meet us weekly until this works out.
• We have to figure out what we're doing to know what we need. We should do that soon.

Friday - 30 May 2008 - 2:30PM - CIS E125

Who should be there: Damon, meeting Sean Crosson to discuss Caulobacter as an expression vector

Crosson has all the plasmids and the Caulobacter and the experience, and will share it all; he has not worked with RsaA secretion personally but he hears it's robust. The reported ridiculously high transcript strength is due also to the stability of the mRNA, not just to strong expression, and that stability may not be conferred to a signal-target fusion mRNA. Caulobacter is really easy to culture and electroporate, and secretes a scum of solid protein that can be skimmed off the top of the culture medium, but that scum has the target covalently fused to a signal sequence. Crosson thinks expression and secretion will be trivial, but cleaving off the secretion signal might take more work.

Next actions:

1. Find some way to make available on this wiki the protocols Crosson photocopied for me

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