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Team:Paris/August 15
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=='''Transformation of the ligations we did [[Team:Paris/August 14| yesterday]]'''== | =='''Transformation of the ligations we did [[Team:Paris/August 14| yesterday]]'''== | ||
| - | We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]]. | + | We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells <br>[[Team:Paris/Notebook/Protocols#Transformation|standard protocol]]. |
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| + | |||
| + | ==Digestion== | ||
| + | |||
| + | ===Measurement of concentration of minipreps=== | ||
| + | [[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]] | ||
| + | |||
| + | {| border="1" style="text-align: center" | ||
| + | |Digestion | ||
| + | |Miniprep used | ||
| + | |Concentration (µg/mL) | ||
| + | |ratio 260/280 | ||
| + | |- | ||
| + | |D146 | ||
| + | |MP148.2 | ||
| + | |123 | ||
| + | |1.58 | ||
| + | |- | ||
| + | |D147 | ||
| + | |MP153.3 | ||
| + | |113 | ||
| + | |1.68 | ||
| + | |} | ||
| + | |||
| + | |||
| + | ===Digestion=== | ||
| + | [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] | ||
| + | |||
| + | |||
| + | ===Electrophoresis for extraction=== | ||
| + | [[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]] | ||
Revision as of 14:09, 15 August 2008
Transformation of the ligations we did yesterdayWe transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
DigestionMeasurement of concentration of minipreps
Digestion
Electrophoresis for extraction |
