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Team:MIT/Colony PCR
From 2008.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
2 uL primer #1 (to 10uM) | 2 uL primer #1 (to 10uM) | ||
| - | |||
2 uL primer #2 | 2 uL primer #2 | ||
| - | |||
0.4 uL DNTPs | 0.4 uL DNTPs | ||
| - | |||
2 uL Buffer | 2 uL Buffer | ||
| - | |||
5 uL colony culture | 5 uL colony culture | ||
| - | |||
0.2 uL Taq | 0.2 uL Taq | ||
| - | |||
10.4 uL H20 | 10.4 uL H20 | ||
Latest revision as of 20:13, 15 August 2008
Colony PCR
2 uL primer #1 (to 10uM) 2 uL primer #2 0.4 uL DNTPs 2 uL Buffer 5 uL colony culture 0.2 uL Taq 10.4 uL H20
92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00
