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Team:University of Lethbridge/Notebook/GeneralLabAugust
From 2008.igem.org
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===Augest 13, 2008=== | ===Augest 13, 2008=== | ||
| - | ==== | + | ====Nathan Puhl and Sebastian==== |
| - | + | Verified and quantified the LacI and DT restriction digest products. | |
Set up a Ligase experiment for LacI and DT | Set up a Ligase experiment for LacI and DT | ||
| Line 11: | Line 11: | ||
- 7.2 uL ddH2O | - 7.2 uL ddH2O | ||
- 20 uL total volume | - 20 uL total volume | ||
| + | |||
| + | ===August 16, 2008=== | ||
| + | ====Roxanne==== | ||
| + | |||
| + | Used Qiagen Plasmid MiniPrep Kit to Plasmid Prep Last Year's Biobrick Parts that were Incubated overnight. | ||
| + | |||
| + | Ran Plasmids in a 1% Agarose Gel at 100 V for 30 minutes. | ||
Revision as of 00:25, 16 August 2008
Contents |
Augest 13, 2008
Nathan Puhl and Sebastian
Verified and quantified the LacI and DT restriction digest products.
Set up a Ligase experiment for LacI and DT
- 1.3 uL vector(DT) - 8 uL insert(LacI) - 2 uL 10x Ligase buffer - 1.5 uL Ligase - 7.2 uL ddH2O - 20 uL total volume
August 16, 2008
Roxanne
Used Qiagen Plasmid MiniPrep Kit to Plasmid Prep Last Year's Biobrick Parts that were Incubated overnight.
Ran Plasmids in a 1% Agarose Gel at 100 V for 30 minutes.
