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NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Contents 1 Characterization of LacI-GFP 1.1 Results obtained at 37 degree Celcius 1.2 RFU Measurements After 3 hours 1.2.1 For Lactose induction of 0 to 10 mM 1.2.2 In comparision with the Standards 1.3 RFU Measurements After 3.5 hours 1.3.1 For Lactose induction of 0 to 10 mM 1.3.2 In comparision with the Standards 1.4 Image Results obtained from fluorescence microscope

Characterization of LacI-GFP

Results obtained at 37 degree Celcius

RFU Measurements After 3 hours

For Lactose induction of 0 to 10 mM

In comparision with the Standards

RFU Measurements After 3.5 hours

For Lactose induction of 0 to 10 mM

In comparision with the Standards

Image Results obtained from fluorescence microscope

The above picture shows the green fluorescence produced by Top 10 ecoli cells under blue light excitation.