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Team:Chiba/Calendar-Home/17 August 2008
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(New page: ===Toggle swich team=== バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026 Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) Insert PCR <br>DNA template ...)
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(New page: ===Toggle swich team=== バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026 Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) Insert PCR <br>DNA template ...)
Newer edit →
Revision as of 08:43, 17 August 2008
Toggle swich team
バイオブリックパーツおこし BBa_I73002 BBa_I73004 BBa_I73003 BBa_I763026
Elution :EB buffer 5μL -> Spin(15000rpm,3Min.)
Insert PCR
DNA template 1μL
Fwd primer(VF II) 5μL
Rev primer(VR) 5μL
Termo pol buffer 10μL
Vent pol 1μL
dH2O 68μL
==========================
Total:100μL
95℃(5min.) -> [95℃(30sec.) -> 53℃(1min.) -> 72℃(2min.)]x25cycle -> 72℃(10min.) -> store
Gel check
