"
Team:BCCS-Bristol/Protocols-DMSO stocks
From 2008.igem.org
(Difference between revisions)
(New page: <html><link rel="stylesheet" href="http://www.chofski.co.uk/iGEM/bccs-igem.css" type="text/css"></html> __NOTOC__ <div class="bccsNavBar"> {| align="center" !align="center"|[[Team:BCCS-Br...) |
Tgorochowski (Talk | contribs) |
||
| Line 25: | Line 25: | ||
# Store the DMSO culture in the -80°C freezer | # Store the DMSO culture in the -80°C freezer | ||
# In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok | # In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok | ||
| + | |||
| + | <br> | ||
| + | </div> | ||
Revision as of 19:45, 18 August 2008
DMSO stocks
- Make an overnight culture of the bacteria in LB broth (If the strain carries a resistance gene on a plasmid, you have to use LB with antibiotics)
- Next morning, put 70 µl DMSO into a cryotube that you labelled with the strain’s name and the date
- Add 930 µl overnight culture
- Incubate immediately for 30 min on ice
- Store the DMSO culture in the -80°C freezer
- In the evening (or in one of the following days), make a streak out of the stock to see whether the bacteria are ok
