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Team:The University of Alberta/18 August 2008
From 2008.igem.org
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(New page: ==Today== *Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEV...) |
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*Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEVER, there was NO growth on the binar vector plates! What's going on? | *Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEVER, there was NO growth on the binar vector plates! What's going on? | ||
**It might be that the vector is simply too large to transform by heatshock into the cells (its nearlt 7kb!). We're going to try a regular transformation to see how if it works. If not, James suggested we try electroporation. | **It might be that the vector is simply too large to transform by heatshock into the cells (its nearlt 7kb!). We're going to try a regular transformation to see how if it works. If not, James suggested we try electroporation. | ||
| + | *Retransformed pBS1A3 since it didnt work last time | ||
Latest revision as of 21:54, 18 August 2008
Today
- Site Directed Mutagenesis: Got LOTS of growth on the transformation control, a fair amount of growth on the mutagenesis control that was positive so we know that it works! HOWEVER, there was NO growth on the binar vector plates! What's going on?
- It might be that the vector is simply too large to transform by heatshock into the cells (its nearlt 7kb!). We're going to try a regular transformation to see how if it works. If not, James suggested we try electroporation.
- Retransformed pBS1A3 since it didnt work last time
