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EPF-Lausanne/12 August 2008
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We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp). | We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp). | ||
| - | We decide to | + | We suspect the newest mini-prep to be bad, so we decide to do a new digestion tommorrow, using both the E1010 and F1610 that we used for positive control and those of an old mini-prep we have left. |
==R0040/B0034 and R0071/B0034== | ==R0040/B0034 and R0071/B0034== | ||
We do the transformation. | We do the transformation. | ||
Revision as of 09:09, 19 August 2008
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Molecular Biology, assemblage of biobricks
All cells culture worked but unfortunately, there seems to have been a problem in the mini-prep procedure, as none of them have more than 30 ng/ul of DNA. So we redo new cell cultures once more.
F1610 and E1010
We do a gel to check if the ligation has worked (we cut the plasmids with SpeI and EcoRI to see the size of the insert). Unfortunately we don't see any fragment around the expected 1500 bp. However the positive control doesn't work either. The positive controls are F1610 (800 bp) and E1010 (700 bp). We suspect the newest mini-prep to be bad, so we decide to do a new digestion tommorrow, using both the E1010 and F1610 that we used for positive control and those of an old mini-prep we have left.
R0040/B0034 and R0071/B0034
We do the transformation.
