"
Team:Paris/August 19
From 2008.igem.org
(Difference between revisions)
(→Screening of the cloning of E0240 and FlhDC+promotor) |
(→Screening of the cloning of E0240 and FlhDC+promotor) |
||
| Line 35: | Line 35: | ||
In order to check these 2 hypothesis and to isolate (if it is possible) the right clone (containing the plasmid with the insert). We decided to spread the "clone" in question in a LB plate in order to carry out a PCR screening on single colonies. | In order to check these 2 hypothesis and to isolate (if it is possible) the right clone (containing the plasmid with the insert). We decided to spread the "clone" in question in a LB plate in order to carry out a PCR screening on single colonies. | ||
*Take of some bacteria from the glycerol stock | *Take of some bacteria from the glycerol stock | ||
| - | *Resuspension in 400 | + | *Resuspension in 400 µL of LB+antibiotic |
| - | *Spreading of 200 | + | *Spreading of 200 µL in a LB agar plate containing the appropriate antibiotic |
*Incubation overnight at 37°C | *Incubation overnight at 37°C | ||
Revision as of 15:06, 19 August 2008
|
Ligation FCScreening of the cloning of E0240 and FlhDC+promotor
The PCR screening of the transformants L139 and L142 of august 15th revealed several bands for a given clone including one band appearing at the right size.
In order to check these 2 hypothesis and to isolate (if it is possible) the right clone (containing the plasmid with the insert). We decided to spread the "clone" in question in a LB plate in order to carry out a PCR screening on single colonies.
|
