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EPF-Lausanne/20 August 2008
From 2008.igem.org
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| - | =Purification of LacIM= | + | ==PCR of the small parts== |
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| + | We found out that the primers used for PCR are very prominent in the gel. Therefore we cannot purify the small parts from it, as it is not possible to separate them. We will have to keep the small parts in the plasmid and add the other parts into it as was our initial plan. | ||
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| + | ==Purification of LacIM== | ||
| + | |||
After having controlled that the PCR of Ron Weiss's plasmids has worked, we did a gel purification of each form of LacIM separately. We then did another PCR of these products, which we will digest tomorrow. | After having controlled that the PCR of Ron Weiss's plasmids has worked, we did a gel purification of each form of LacIM separately. We then did another PCR of these products, which we will digest tomorrow. | ||
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Revision as of 07:54, 21 August 2008
PCR of the small parts
We found out that the primers used for PCR are very prominent in the gel. Therefore we cannot purify the small parts from it, as it is not possible to separate them. We will have to keep the small parts in the plasmid and add the other parts into it as was our initial plan.
Purification of LacIM
After having controlled that the PCR of Ron Weiss's plasmids has worked, we did a gel purification of each form of LacIM separately. We then did another PCR of these products, which we will digest tomorrow.
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