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Team:Paris/Modeling/f1
From 2008.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
|value | |value | ||
|- | |- | ||
| - | |[ | + | |[expr(pTet)] |
| - | |expression rate of <br> | + | |expression rate of <br> pTet '''with RBS E0032''' |
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| - | | | + | |see [[Paris:Team/Modeling/Programs|"findparam"]] <br> need for 20 measures |
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
| Line 39: | Line 39: | ||
|GFP concentration at steady-state | |GFP concentration at steady-state | ||
|nM | |nM | ||
| - | | | + | |need for 20 measures |
|- | |- | ||
|(''fluorescence'') | |(''fluorescence'') | ||
|value of the observed fluorescence | |value of the observed fluorescence | ||
|au | |au | ||
| - | | | + | |need for 20 measures |
|- | |- | ||
|''conversion'' | |''conversion'' | ||
| Line 50: | Line 50: | ||
|nM.au<sup>-1</sup> | |nM.au<sup>-1</sup> | ||
|(1/79.429) | |(1/79.429) | ||
| + | |} | ||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |param | ||
| + | |signification <br> corresponding parameters in the [[Team:Paris/Modeling/hill_approach/FIFO#resulting_equations|equations]] | ||
| + | |unit | ||
| + | |value | ||
| + | |- | ||
| + | |β<sub>tet</sub> | ||
| + | |production rate of pTet '''with RBS E0032''' <br> β<sub>1</sub> | ||
| + | |nM.s<sup>-1</sup> | ||
| + | | | ||
| + | |- | ||
| + | |(K<sub>tet</sub>/{coef<sub>tetR</sub>}<sup>n<sub>tet</sub></sup>) | ||
| + | |activation constant of pTet <br> K<sub>18</sub> | ||
| + | |nM<sup>n<sub>tet</sub></sup> | ||
| + | | | ||
| + | |- | ||
| + | |n<sub>tet</sub> | ||
| + | |complexation order of pTet<br> of n<sub>18</sub> | ||
| + | |no dimension | ||
| + | | | ||
| + | |- | ||
| + | |K<sub>aTc</sub> | ||
| + | |complexation constant aTc-TetR <br> K<sub>17</sub> | ||
| + | |nM<sup>n<sub>aTc</sub></sup> | ||
| + | | | ||
| + | |- | ||
| + | |n<sub>aTc</sub> | ||
| + | |complexation order aTc-TetR <br> n<sub>17</sub> | ||
| + | |no dimension | ||
| + | | | ||
|} | |} | ||
Revision as of 13:51, 21 August 2008
so, with
that gives, after reducing the expression
with the previous expression of [TetR]eq
so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")
| param | signification | unit | value |
| [expr(pTet)] | expression rate of pTet with RBS E0032 | nM.s-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βtet | production rate of pTet with RBS E0032 β1 | nM.s-1 | |
| (Ktet/{coeftetR}ntet) | activation constant of pTet K18 | nMntet | |
| ntet | complexation order of pTet of n18 | no dimension | |
| KaTc | complexation constant aTc-TetR K17 | nMnaTc | |
| naTc | complexation order aTc-TetR n17 | no dimension |
