Team:Paris/Modeling/f1

From 2008.igem.org

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Revision as of 13:55, 21 August 2008

F1a.jpg
In the exponential phase of growth, at the steady state, we have
TetReqtot.jpg

so, with

F1expr.jpg

that gives, after reducing the expression

F1exprredu.jpg

with the previous expression of [TetR]eq

F1exprreduf0.jpg

so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")

F1exprCalc.jpg


param signification unit value
[expr(pTet)] expression rate of
pTet with RBS E0032
nM.s-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
βtet production rate of pTet with RBS E0032
β1
nM.s-1
(Ktet/{coeftetR}ntet) activation constant of pTet
K18
nMntet
ntet complexation order of pTet
n18
no dimension
KaTc complexation constant aTc-TetR
K17
nMnaTc
naTc complexation order aTc-TetR
n17
no dimension