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EPF-Lausanne/21 August 2008
From 2008.igem.org
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==PCR of Ron Weiss plasmid== | ==PCR of Ron Weiss plasmid== | ||
| - | We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product | + | We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. |
| + | We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel. | ||
==Ligations== | ==Ligations== | ||
Revision as of 14:34, 21 August 2008
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Purifying LacIM
We obtained bad results -> No band visible on the gel. We are then starting again...
PCR of Ron Weiss plasmid
We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel.
