Team:Paris/Modeling/f6

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[[Image:f6DCA.png|900px]]
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[[Image:f6DCA.png|thumb]] At the steady-state, we have
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<center>[[Image:FlhDCeq.jpg]]</center>
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and
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<center>[[Image:FliAeq.jpg]]</center>
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so the expression
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<center>[[Image:f6expr.jpg]]</center>
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gives
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<center>[[Image:f6FlhDC.jpg]]</center>
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and
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<center>[[Image:f6FliA.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f6FlhDCCalc.jpg]]</center>
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and
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<center>[[Image:f6FliACalc.jpg]]</center>
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification
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|unit
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|value
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|-
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|[expr(pFliL)]
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|expression rate of <br> pFliA '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|s<sup>-1</sup>
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|ln(2)/3600
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|-
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]]
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|unit
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|value
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|-
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|β<sub>53</sub>
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|production rate of FlhDC-pFliL '''with RBS E0032''' <br> β<sub>53</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>47</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>47</sub></sup>)
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|activation constant of FlhDC-pFliL <br> K<sub>47</sub>
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|nM<sup>n<sub>47</sub></sup>
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|
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|-
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|n<sub>47</sub>
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|complexation order of FliA-pFliL <br> n<sub>47</sub>
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|no dimension
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|
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|-
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|-
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|β<sub>54</sub>
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|production rate of FliA-pFliL '''with RBS E0032''' <br> β<sub>54</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>48</sub>/{coef<sub>fliA</sub>}<sup>n<sub>48</sub></sup>)
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|activation constant of OmpR-pFlhDC <br> K<sub>48</sub>
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|nM<sup>n<sub>48</sub></sup>
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|
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|-
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|n<sub>48</sub>
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|complexation order of OmpR-pFlhDC <br> n<sub>48</sub>
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|no dimension
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|
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|}
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<br><br>
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Then, if we have time, we want to verify the expected relation
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<center>[[Image:SumpFlgA.jpg]]</center>

Revision as of 16:15, 21 August 2008

F6DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F6expr.jpg

gives

F6FlhDC.jpg

and

F6FliA.jpg

and for calculated values of the TF,

F6FlhDCCalc.jpg

and

F6FliACalc.jpg



param signification unit value
[expr(pFliL)] expression rate of
pFliA with RBS E0032 		nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
β53 production rate of FlhDC-pFliL with RBS E0032
β53 		nM.s-1
(K47/{coefflhDC}n47) activation constant of FlhDC-pFliL
K47 		nMn47
n47 complexation order of FliA-pFliL
n47 		no dimension
β54 production rate of FliA-pFliL with RBS E0032
β54 		nM.s-1
(K48/{coeffliA}n48) activation constant of OmpR-pFlhDC
K48 		nMn48
n48 complexation order of OmpR-pFlhDC
n48 		no dimension



Then, if we have time, we want to verify the expected relation

SumpFlgA.jpg
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