Team:BCCS-Bristol/Protocols-Media

From 2008.igem.org

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===LB broth or agar (200 ml)===
===LB broth or agar (200 ml)===
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{| {{table}}
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{| class="bccstable2"
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
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===Swimming agar medium ===
===Swimming agar medium ===
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{| {{table}}
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{| class="bccstable2"
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
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===SOC medium===
===SOC medium===
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{| {{table}}
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{|class="bccstable2"
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Component'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''
| align="center" style="background:#f0f0f0;"|'''Concentration [%]'''

Revision as of 08:54, 25 August 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous


Media

LB broth or agar (200 ml)

Component Concentration [%] Amount for 200 ml
Yeast Extract0.51 g
Bactotryptone12 g
NaCl12 g
AgarFor plates 2For plates 4 g

Swimming agar medium

Component Concentration [%] Amount for 200 ml
Bactotryptone12 g
NaCl0.52 g
Agarvarious

SOC medium

Component Concentration [%] Amount for 95 (100) ml
Bactotryptone22 g
Bacto yeast extract0.50.5 g
NaCl0.050.05 g

Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):

• 0.5 ml 2 M MgCl2

• 2 ml 1 M Glucose


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