Team:BCCS-Bristol/Protocols-Media

From 2008.igem.org

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| align="center" style="background:#f0f0f0;"|'''Amount for 200 ml'''
| align="center" style="background:#f0f0f0;"|'''Amount for 200 ml'''
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Revision as of 08:55, 25 August 2008

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Media

LB broth or agar (200 ml)

Component Concentration [%] Amount for 200 ml
Yeast Extract0.51 g
Bactotryptone12 g
NaCl12 g
AgarFor plates 2For plates 4 g

Swimming agar medium

Component Concentration [%] Amount for 200 ml
Bactotryptone12 g
NaCl0.52 g
Agarvarious

SOC medium

Component Concentration [%] Amount for 95 (100) ml
Bactotryptone22 g
Bacto yeast extract0.50.5 g
NaCl0.050.05 g

Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):

• 0.5 ml 2 M MgCl2

• 2 ml 1 M Glucose


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