"
Team:BCCS-Bristol/Protocols-Media
From 2008.igem.org
(Difference between revisions)
Tgorochowski (Talk | contribs) (→SOC medium) |
Tgorochowski (Talk | contribs) (→SOC medium) |
||
| Line 63: | Line 63: | ||
Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized): | Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized): | ||
| - | + | * 0.5 ml 2 M MgCl2 | |
| - | + | * 2 ml 1 M Glucose | |
| - | + | ||
| - | + | ||
<br> | <br> | ||
</div> | </div> | ||
Revision as of 08:56, 25 August 2008
Media
LB broth or agar (200 ml)
| Component | Concentration [%] | Amount for 200 ml |
| Yeast Extract | 0.5 | 1 g |
| Bactotryptone | 1 | 2 g |
| NaCl | 1 | 2 g |
| Agar | For plates 2 | For plates 4 g |
Swimming agar medium
| Component | Concentration [%] | Amount for 200 ml |
| Bactotryptone | 1 | 2 g |
| NaCl | 0.5 | 2 g |
| Agar | various |
SOC medium
| Component | Concentration [%] | Amount for 95 (100) ml |
| Bactotryptone | 2 | 2 g |
| Bacto yeast extract | 0.5 | 0.5 g |
| NaCl | 0.05 | 0.05 g |
Mix the three components with 95 ml distilled water and autoclave it. Just before using, add (filter sterilized):
- 0.5 ml 2 M MgCl2
- 2 ml 1 M Glucose
