Imperial College/19 August 2008
From 2008.igem.org
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==Wetlab== | ==Wetlab== | ||
===''B.subtilis''=== | ===''B.subtilis''=== | ||
- | *Transformation protocol 2 was repeated today, specifically the competent cells were prepared. Alterations to the previous attempt at this protocol was to use a greater O.D.<sub>600</sub>, at 1.8-1.9 as opposed to 1.5. This is to help stress them more and improve competence. Fingers crossed for tomorrow! | + | *Transformation protocol 2 was repeated today, [[http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol]] |
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+ | specifically the competent cells were prepared. Alterations to the previous attempt at this protocol was to use a greater O.D.<sub>600</sub>, at 1.8-1.9 as opposed to 1.5. This is to help stress them more and improve competence. Fingers crossed for tomorrow! | ||
== Miscellaneous == | == Miscellaneous == | ||
* Krupa's birthday today! She's 19, for the third time. [: | * Krupa's birthday today! She's 19, for the third time. [: |
Revision as of 14:46, 25 August 2008
19 August 2008WetlabB.subtilis
specifically the competent cells were prepared. Alterations to the previous attempt at this protocol was to use a greater O.D.600, at 1.8-1.9 as opposed to 1.5. This is to help stress them more and improve competence. Fingers crossed for tomorrow! Miscellaneous
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