EPF-Lausanne/25 August 2008
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+ | |||
+ | ==Ligations== | ||
+ | |||
+ | ===SL1=== | ||
+ | |||
+ | Vector: B0015 (Term) | ||
+ | |||
+ | Insert: LacIM (from pHD1) | ||
+ | |||
+ | => Nothing grew on the plates, we suppose because PCR+purification from the Ron Weiss Plasmid (pHD1) didn't yield enough insert DNA. | ||
+ | |||
+ | ===KL1=== | ||
+ | |||
+ | Vector: B0034 (RBS) | ||
+ | |||
+ | Insert: E1010 (RFP) | ||
+ | |||
+ | => Negative Control Plate had colonies. |
Revision as of 12:15, 26 August 2008
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Ligations
SL1
Vector: B0015 (Term)
Insert: LacIM (from pHD1)
=> Nothing grew on the plates, we suppose because PCR+purification from the Ron Weiss Plasmid (pHD1) didn't yield enough insert DNA.
KL1
Vector: B0034 (RBS)
Insert: E1010 (RFP)
=> Negative Control Plate had colonies.