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EPF-Lausanne/21 August 2008
From 2008.igem.org
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We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. | We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. | ||
We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel. | We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel. | ||
| + | |||
| + | ==Ligations== | ||
| + | |||
| + | The ligations worked! | ||
| + | |||
| + | We have now: | ||
| + | |||
| + | ===GL=== | ||
| + | I14033 + I1466 | ||
| + | |||
| + | ===VL=== | ||
| + | C0040 + B0015 | ||
Latest revision as of 12:24, 26 August 2008
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Contents |
Purifying LacIM
We obtained bad results -> No band visible on the gel. We are then starting again...
PCR of Ron Weiss plasmid
We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel.
Ligations
The ligations worked!
We have now:
GL
I14033 + I1466
VL
C0040 + B0015
