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User:University of Washington/28 August 2008
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| + | ==LuxR from AraC and TetR(Faifan)== | ||
| + | |||
| + | - ligation | ||
| + | *mix | ||
| + | |||
| + | |||
| + | *incubate at room temp 45 mins | ||
| + | *denature at 65 degree Celsius 10 mins | ||
| + | *filtered 20 mins | ||
| + | |||
| + | - transformed 2ul DNA into 45 ul XL1-Blue | ||
| + | |||
| + | - inoculated on CAM plates | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 19:40, 28 August 2008
LuxR from AraC and TetR(Faifan)
- ligation
- mix
- incubate at room temp 45 mins
- denature at 65 degree Celsius 10 mins
- filtered 20 mins
- transformed 2ul DNA into 45 ul XL1-Blue
- inoculated on CAM plates
Back to Team:University_of_Washington/Notebook#Notebook
