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User:University of Washington/28 August 2008
From 2008.igem.org
(Difference between revisions)
(→LuxR from AraC and TetR(Faifan)) |
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| Line 3: | Line 3: | ||
- ligation | - ligation | ||
*mix | *mix | ||
| - | + | <table border=1> | |
| - | + | <tr align="center"> | |
| + | <th></th><th>dH20(ul)</th><th>Buffer(ul)</th><th>promoter(ul)</th><th>GFP(ul)</th><th>LuxR(ul)</th><th>T4 ligase(ul)</th> | ||
| + | </tr> | ||
| + | <tr align="center"> | ||
| + | <td>LuxR</td><td>12.34</td><td>2</td><td>1.54</td><td>-</td><td>3.12</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr align="center"> | ||
| + | <td>GFP</td><td>13.84</td><td>2</td><td>1.54</td><td>1.62</td><td>-</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr align="center"> | ||
| + | <td>no insert</td><td>15.46</td><td>2</td><td>1.54</td><td>-</td><td>-</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr align="center"> | ||
| + | <td>no T4 ligase</td><td>14.84</td><td>2</td><td>1.54</td><td>1.62</td><td>-</td><td>-</td> | ||
| + | </tr> | ||
| + | </table> | ||
*incubate at room temp 45 mins | *incubate at room temp 45 mins | ||
*denature at 65 degree Celsius 10 mins | *denature at 65 degree Celsius 10 mins | ||
Latest revision as of 19:45, 28 August 2008
LuxR from AraC and TetR(Faifan)
- ligation
- mix
| dH20(ul) | Buffer(ul) | promoter(ul) | GFP(ul) | LuxR(ul) | T4 ligase(ul) | |
|---|---|---|---|---|---|---|
| LuxR | 12.34 | 2 | 1.54 | - | 3.12 | 1 |
| GFP | 13.84 | 2 | 1.54 | 1.62 | - | 1 |
| no insert | 15.46 | 2 | 1.54 | - | - | 1 |
| no T4 ligase | 14.84 | 2 | 1.54 | 1.62 | - | - |
- incubate at room temp 45 mins
- denature at 65 degree Celsius 10 mins
- filtered 20 mins
- transformed 2ul DNA into 45 ul XL1-Blue
- inoculated on CAM plates
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