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Template:Team:UC Berkeley/Notebook/MJV construction
From 2008.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
mv002 Reverse Biobricking of <Cre! | mv002 Reverse Biobricking of <Cre! | ||
cgttaGGATCCttaatcgccatcttccagcaggcg | cgttaGGATCCttaatcgccatcttccagcaggcg | ||
| + | </pre> | ||
| + | |||
| + | '''Construction of Cre> Basic Part K112101''' | ||
| + | <pre> | ||
| + | PCR mv003/mv004 on pBca9145-Bca1117 (1060bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112101 | ||
| + | ----------------------------------------------- | ||
| + | mv003 Forward Biobricking of Cre> | ||
| + | ggataGAATTCgtgAGATCTatgtccaatttactgaccgtac | ||
| + | mv004 Reverse Biobricking of Cre> | ||
| + | cgttaGGATCCatcgccatcttccagcaggcg | ||
| + | </pre> | ||
| + | |||
| + | |||
| + | '''Construction of <Cre> Basic Part K112102''' | ||
| + | <pre> | ||
| + | PCR mv001/mv004 on pBca9145-Bca1117 (1057bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112102 | ||
| + | ----------------------------------------------- | ||
| + | mv001 Forward Biobricking of <Cre! | ||
| + | ggataGAATTCgtgAGATCTtccaatttactgaccgtacac | ||
| + | mv004 Reverse Biobricking of Cre> | ||
| + | cgttaGGATCCatcgccatcttccagcaggcg | ||
</pre> | </pre> | ||
Revision as of 22:59, 4 June 2008
Construction of <Cre! Basic Part K112100
PCR mv001/mv002 on pBca9145-Bca1117 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100 ----------------------------------------------- mv001 Forward Biobricking of <Cre! ggataGAATTCgtgAGATCTtccaatttactgaccgtacac mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagcaggcg
Construction of Cre> Basic Part K112101
PCR mv003/mv004 on pBca9145-Bca1117 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112101 ----------------------------------------------- mv003 Forward Biobricking of Cre> ggataGAATTCgtgAGATCTatgtccaatttactgaccgtac mv004 Reverse Biobricking of Cre> cgttaGGATCCatcgccatcttccagcaggcg
Construction of <Cre> Basic Part K112102
PCR mv001/mv004 on pBca9145-Bca1117 (1057bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112102 ----------------------------------------------- mv001 Forward Biobricking of <Cre! ggataGAATTCgtgAGATCTtccaatttactgaccgtacac mv004 Reverse Biobricking of Cre> cgttaGGATCCatcgccatcttccagcaggcg
