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Team:Caltech/Project/Vitamins
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The experimental setup included running a standard curve with known amounts of folic acid (0-10ng) simultaneously with the samples, in order to have the same basis for comparison. The hope was that the standard curve growth OD values would correspond with known concentrations, and so sample concentrations could be interpolated based upon the standard. For ''folKE'' and ''folBKE,'' which were in constitutive high-copy plasmids, these genes were tested with and without the addition of 100ng of pABA during inoculation. ''folB'', which is in an inducible copy plasmid, it was tested induced high-copy as well as uninduced low-copy, with and without 100ng of pABA during inoculation. Folate levels were assayed based on protocol mentioned above[https://2008.igem.org/Team:Caltech/Protocols/Folate_assay]. | The experimental setup included running a standard curve with known amounts of folic acid (0-10ng) simultaneously with the samples, in order to have the same basis for comparison. The hope was that the standard curve growth OD values would correspond with known concentrations, and so sample concentrations could be interpolated based upon the standard. For ''folKE'' and ''folBKE,'' which were in constitutive high-copy plasmids, these genes were tested with and without the addition of 100ng of pABA during inoculation. ''folB'', which is in an inducible copy plasmid, it was tested induced high-copy as well as uninduced low-copy, with and without 100ng of pABA during inoculation. Folate levels were assayed based on protocol mentioned above[https://2008.igem.org/Team:Caltech/Protocols/Folate_assay]. | ||
| - | For Test #1, | + | For Test #1, we tested only ''folKE'' and ''folBKE'', as ''folB'' needed a longer incubation time. The standard curve and growth results are shown on the right. First off, it is clear that the standard curve is pretty much useless in terms of linearity and relative growth compared to the samples. Although the reasons for this were unclear, the growth data for the samples are very encouraging since the relative growth levels match what we would expect. We see that the addition of 100 ng of pABA during inoculation dramatically increases overall folate levels for both ''folKE'' and ''folBKE'' relative to the samples without pABA. Furthermore, adding pABA to the wild type controls did not affect growth at all, suggesting that the assay bacteria ''L. rhamnosus'' was not metabolizing the extra pABA. |
===para-Aminobenzoic Acid (pABA) HPLC Assay Results for ''pabA'' and ''pabB''=== | ===para-Aminobenzoic Acid (pABA) HPLC Assay Results for ''pabA'' and ''pabB''=== | ||
Revision as of 19:25, 31 August 2008
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Background Information on Folate Folate, the generic term for the various forms of Vitamin B9, is an essential vitamin because it is heavily involved in amino acid synthesis as well as single-carbon transfer reactions. Folate deficiencies in women can result in birth defects such as neural tube defects and other spinal cord malformations. As important as folate is, humans are unable to produce folate, and so must obtain it from eating foods such as green leafy vegetables or folate-fortified cereals sybesma1. An engineered strain of bacteria that would constantly release folate into the gut would reduce the need to fortify breads and cereals with folate, as well as reduce folate-related birth defects in regions with little access to folate-containing foods. In addition to all the reasons stated above, folate is an ideal vitamin to be produced in the gut because, unlike many other vitamins, it has been shown to be absorbed in physiologically relevant quantities in the large intestineasrar. Structurally, folate consists of three main parts: pteridine, p-aminobenzoic acid (pABA), and L-glutamate.
Folate Biosynthesis Pathway The folate gene cluster from L.lactis. Black arrows represent genes which have been tested in metabolic engineering studies, shaded arrows represent genes involved in folate biosynthesis, and white arrows represent genes not involved in folate synthesis. sybesma1 Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folAsybesma1. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. We’ve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producerswegkamp1. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the additional benefit of removing the operon from its natural regulatory context.  Our strategy is to clone the entire folate operon from the L.lactis genome and to transform the entire operon into E.coli. However, because we are unsure of whether or not the ribosomal binding sites (RBS) within the L.lactis operon would work in E.coli, we are also going to unpack the operon by cloning each of the four genes individually, placing them behind E.coli RBSs, and then recombine them into a single empty BioBricks™ plasmid. In addition to the main folate operon, we will also be experimenting with overexpression of the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. Wegkamp et al. have shown that in order to increase overall total levels of folate, both the pABA synthesis genes and certain folate production genes need to be overexpressedwegkamp2. The pABA pathway involves three genes, pabA, pabB, and pabC – though in L.lactis, pabB is actually a fusion gene encoding for both pabB and pabCwegkamp2. System Design  The overall system design for testing folate production in E.coli is to construct two plasmids – one for the folate biosynthesis pathway, and one for the pABA synthesis pathway. In addition to ensuring that the plasmids are complementary, each plasmid would need to contain a different variable copy origin of replication, which would be low copy by default, but can be switched to high copy via the addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG)to the media. This will allow us to test overexpression of each plasmid separately. In addition, each plasmid will contain a constitutive promoter, since we would want folate to be produced constantly. The purple dots represent ribosomal binding sites (RBS), followed by the gene (green arrow), and eventually terminating in a double stop (TAATAA) sequence, as regulated by the Registry of Standard Biological Parts. Folate Detection Methods We will be detecting folate production, and thus the relative success of our engineering efforts, via a microbiological assay involving the folate-dependent strain Lactobacillus rhamnosushorne. This assay involves first the characterization of a standard growth curve of L. rhamnosus given known quantities of folate present in the growth media. Once the standard curve has been established, then experimental growth levels, as quantified by spectrophotometry, can be interpolated. PABA concentrations will be measured via high performance liquid chromatography (HPLC) wegkamp2. Folate Microbiological Assay Protocol para-aminobenzoic acid (pABA) HPLC protocol ResultsModifications to System Design We were unable to successfully use PCR to clone out several of our goals: the entire folate gene cluster, folP, and folC. After analyzing the sequencing results of our other folate biosynthesis genes, we realized that the genetic sequences had several point mutations which indicated that we had a homologous, but not identical, genomic DNA than the one that we had based our PCR primer design off of. We believe that this homologous sequence could be the reason why we were unable to successfully extract folP, folC, and subsequently, the entire folate gene cluster (since folP and folC are the last two genes in the cluster) from the genomic DNA of L. lactis that we had ordered from ATCC. The revised target constructs are shown in the figure to the right. As a result, we continued forward with cloning for the four genes we were able to successfully extract: folB, folKE, pabA, and pabB. We still aimed to create individual constructs of each gene in the IPTG inducible-copy plasmid pSB2K3, as well as constructs with the two folate genes combined and with the two pABA synthesis genes combined. However, we had issues cloning our genes into the pSB2K3 + B0015 terminator construct, and so switched to completing the constructs in the high copy plasmid pSB1AK3 + b0015 terminator. As such, our final constructs are: folB in pSB2K3, and folKE, pabA, folB+folKE, and pabB in pSB1AK3. Although the last two were not yet sequence confirmed, we pushed ahead to testing due to time constraints. The goal was to detect higher folate levels with overexpression of folB, folKE, and folBKE with pABA spiked in. Previous studies on folate overexpression have shown that both folate synthesis and pABA synthesis genes need to be overexpressed simultaneously in order to increase total folate levels Wegkamp. As such, tests were done with and without the spiking in of pABA during the E.coli inoculation. Folate Assay Results for folB, folKE, and folBKE  The experimental setup included running a standard curve with known amounts of folic acid (0-10ng) simultaneously with the samples, in order to have the same basis for comparison. The hope was that the standard curve growth OD values would correspond with known concentrations, and so sample concentrations could be interpolated based upon the standard. For folKE and folBKE, which were in constitutive high-copy plasmids, these genes were tested with and without the addition of 100ng of pABA during inoculation. folB, which is in an inducible copy plasmid, it was tested induced high-copy as well as uninduced low-copy, with and without 100ng of pABA during inoculation. Folate levels were assayed based on protocol mentioned above[1]. For Test #1, we tested only folKE and folBKE, as folB needed a longer incubation time. The standard curve and growth results are shown on the right. First off, it is clear that the standard curve is pretty much useless in terms of linearity and relative growth compared to the samples. Although the reasons for this were unclear, the growth data for the samples are very encouraging since the relative growth levels match what we would expect. We see that the addition of 100 ng of pABA during inoculation dramatically increases overall folate levels for both folKE and folBKE relative to the samples without pABA. Furthermore, adding pABA to the wild type controls did not affect growth at all, suggesting that the assay bacteria L. rhamnosus was not metabolizing the extra pABA. para-Aminobenzoic Acid (pABA) HPLC Assay Results for pabA and pabBRelevant Registry PartsBasic Parts
Construction Intermediates
Composite Parts
References<biblio>
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